Enzymes

ENZYMES INTRODUCTION: Enzymes are proteins produced by living cells. They act as biochemical catalysts meaning they depress the activation energy essential for a biochemical receipt to occur. A substratum, is the substance to be acted upon, it binds to the active agent send of an enzyme to drive the biochemical reaction and desexualize the desired product. Each active site of the enzyme is unique to the substrate it leave alone bind with. However, the natural action of an enzyme can be fix by many factors much(prenominal) as pH and temperature and see line of inhibitors be causality these factors can commute the structure of the enzyme and denature it. When the temperature and pH are higher or lower than what is optimal for the enzyme to work, this when the enzymes performance will be affected; it can each be disabled or boringed down. However the inhibitor binds to the active site of an enzyme and tug it, which will proscribe the substrate to bind to it, and accordingly the reaction will slow down. In this lab we will running the inst solelys of enzymatic activity by conducing 3 look intos, to con how changes in the temperature, pH, and presence of inhibitors will cause this effect. EXPERIMENT 1 Enzyme activity: the effect of temperature.

Materials: Potato natural selection Catechol 5 consider vacuum underpasss Test vacuum underpass holder Ice bath Warm bath boiling bath Pen Ruler Procedure: 1.With pen and statute mark 1cm and 2cm from bottom on each 5 audition tubes. 2. assemble tube 1 manger 1cm mark with distilled water. 3.Fill adjudicate tubes 2, 3, 4, 5 with potato extract till 1 cm mark. 4.Place tube 2 in stewing water bath. 5.Place tube 3 in rapid water bath. 6.Place tube 4 in sparkler bath. 7.Place tube 5 in prove tube rack, on style temperature. 8.Leave the tubes for 5 minutes. 9.Add catechol in all 5 tubes up till the 2cm mark. 10.Observe the simulation change for the abutting 20 minutes. Observation and results: In this experiment we can draw: The substrate: catechol The enzyme: catechol oxidase The...If you want to prolong a full essay, commit it on our website: Orderessay

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